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These tight junction complexes regulate paracellular permeability, selectively restricting the passage of luminal antigens, the gut microbiota, and toxins into the host circulation

10.1128/AAC.01547-05 133 MllerR

A431 and RPE cells were lysed with 1% NP-40 lysis buffer containing 50 mM Tris-HCl at pH 7.5, 150 mM NaCl, 1 mM EGTA, 5 mM ATP, 5 mM MgCl 2 and complete protease inhibitor cocktail ( Roche ), homogenised using a 25-g needle and clarified by centrifugation at 20,000 g for 15 min at 4 C