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Description
In eukaryotes, TML is the product of lysosomal or proteasomal degradation of proteins such as calmodulin, myosin, actin, cytochrome c, and histones, which contain N -methylated lysine residues from post-translational modification (Strijbis et al., 2010)
In the fatty acid oxidation (FAO) assay, the culture medium was washed twice and replaced with substrate-limited DMEM (102353-100, Seahorse Bioscience, Billerica, MA, USA) supplemented with 25 mM d-glucose, 1 mM sodium pyruvate, 0.5 mM l-carnitine hydrochloride (07353-51, Nacalai Tesque, Kyoto, Japan) and 0.5 mM NaOH to adjust to pH 7.4, and the cells were equilibrated for 1 h at 33 C in an incubator (0.03% CO 2 ) prior to the assay
Consegna assicurata 0,90 (eventualmente da aggiungere al costo della consegna con corriere espresso) garantisce in caso di danni o perdita della spedizione, senza attendere che la situazione venga risolta entro il termine legale
doi: 10.1186/s12882-025-04039-w
