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GPx utilizes GSH as a cofactor to reduce H 2 O 2 , resulting in the formation of GSSG, which in turn can be reduced to GSH by GR using the electron donor NADPH [80,81]

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The rate constants from our deglutathionylation assays between 6.4 10 4 and 1.4 10 6 M 1 s 1 are very similar to the rate constants for PfGrx or plant and mammalian glutaredoxins from discontinuous or coupled enzymatic assays with other high and low molecular weight GSSR substrates (Table 1) 9,11,14,19 , thus confirming our assay as an alternative reliable method for the direct analysis of enzyme-catalyzed deglutathionylations

10.1016/j.ygcen.2020.113670 Gen