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Description
Thiamine is most important in the breakdown of pyruvic acid, a waste product in hard working muscles, along with lactic acid
doi: 10.1038/nrm3801
Following these procedures, the cell pellets of each sample were collected by centrifugation at various durations (6, 12, 24, 48 h) and resuspended in binding buffer (20 mM NaH 2 PO 4 , 500 mM NaCl, 30 mM imidazole, pH 7.4)
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