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The development of high-resolution mass spectrometry (HRMS) (Mehmood et al., 2015), data-dependent acquisition (DDA) (Mann et al., 2001), targeted multiple reaction monitoring (MRM) (Picotti and Aebersold, 2012
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For the luciferase assay, HEK293T cells were seeded in 24-well plates until they reached 70% confluency and, subsequently, allocated into five groups: (1) transfected with GL-vector and Flag, (2) transfected with GL-VEGFA-WT and Flag-STAT6, (3) transfected with GL-VEGFA-Mut and Flag-STAT6, (4) transfected with GL-IL8-WT and Flag-STAT6, and (5) transfected with GL-IL8-Mut and Flag-STAT6

Glutathione Injections: Are They Really Safe and Effective