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FIGURE 4 Validation of RNA-seq results and the expression analysis of Wnt/-catenin signaling pathway-related genes To verify the results of RNA-seq, 13 differentially expressed genes, including 6 downregulated and 7 upregulated genes, were randomly selected for qRT-PCR assay

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Mechanistic analyses attribute these benefits directly to improve systemic NO bioavailability

Since the reduction of NS5A hyperphosphorylation indicated changes in replication complex formation, we performed co-immunoprecipitation (Co-IP) to analyse the effect of D1467G on the interaction of NS3 with other NS proteins