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The enzyme dihydrofolate reductase (DHFR) is a key enzyme in cell replication
Ultrathin sections (80 nm thick) were cut with an ultramicrotome (Leica, EMUC6, Germany), and then stained with uranyl acetate and lead citrate and finally examined by a Tecnai G 2 Spirit TWIN transmission electron microscope (FEI, Hillsboro, OR, USA)
This reaction uses betaine (trimethylglycine) as a methyl donor, which is acquired either by dietary uptake, or as a byproduct of choline degradation [21]
