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Determination of testicular glutathione peroxidase (GPX) The activity of the antioxidant enzyme glutathione peroxidase was determined using glutathione reductase and NADPH

All treatments were performed in pre-warmed (37C) differentiation medium with reduced NGF- content (5 ng/ml) or with buffered Hank's solution (Capricorn Scientific GmbH, 5.26 mM KCl, 0.43 mM KH 2 PO 4 , 134.2 mM NaCl, 4.09 mM NaHCO 3 , 0.33 mM Na 2 HPO 4 , 5.44 mM glucose, 2 mM CaCl 2 , and 20 mM HEPES, pH 7.4

TNF- down-regulation by albumin inhibits the activation of pro-inflammatory nuclear factor-kappa B (NF-) pathway51 and recruitment of leukocytes,51,54 further limiting inflammation (Figure 2)

The bodys natural antioxidants, including a molecule called glutathione, are supposed to keep inflammation in check