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The process takes just seconds and causes minimal discomfort
AKT, a signaling molecule of the protein kinase B family, is activated in many human cancers and is part of the phosphatidylinositol 3-kinase pathway
Just because you have the genes doesnt mean you have the disease
Similarly, for overexpression cell lines, gBlocks (IDT) containing the cDNA of interest were cloned into pMXS-IRES-Blast linearized with BamHI and NotI by Gibson Assembly (NEB)
