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Refer to your peptide guide for site-specific recommendations and storage protocols

Beads were washed twice with 1 mL low-salt wash buffer [20 mM Tris-HCl pH 8.0, 150 mM NaCl, 2 mM EDTA, 0.1% (w/v) SDS, 1% (w/v) Triton X-100], twice with 1 mL high-salt wash buffer [20 mM Tris-HCl pH 8.0, 500 mM NaCl, 2 mM EDTA, 0.1% (w/v) SDS, 1% (w/v) Triton X-100], twice with 1 mL LiCl wash buffer [10 mM Tris-HCl pH 8.0, 0.25 M LiCl, 1 mM EDTA, 1% (w/v) sodium deoxycholate, 1% (w/v) Nonidet P-40], and twice with 1 mL TE buffer [10 mM Tris-HCl pH 8.0, 1 mM EDTA]

The current method of -EC production is mainly chemical synthesis from Glu and Cys