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Cy5.5-labelled B-017 (100 nM in 50 mM Tris, 10 mM Glutathione, pH 8.0) was titrated with human BNIP3, BCL-2 and glutathione-S-transferase and the fluorescence anisotropy r ( l ex = 680 nm, l em = 695 nm) was measured for each step

Kwak, M.K

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