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Then, samples were diluted in 4 LDS sample buffer and 10 reducing agent (Invitrogen) and boiled at 95 C for 5 min and loaded onto Bolt 412% Bis-Tris Plus Gels (Invitrogen)
Thus, in addition to the properties of the N=C=S group, the structural diversity of ITC side chains could have a large influence on their biological activities
Wallis, D., Li, K., Lui, H., Hu, K., Chen, M
Identification of the target protein and molecular mechanism of honokiol in anti-inflammatory action
