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Yoshida T, Delafontaine P

Mammalian cell-based assay to identify coronavirus protease inhibitor (SARS-CoV, SARS-CoV-2, and MERS) Expression constructs of SARS-CoV, SARS-CoV-2, or MERS 3CL protease (Twist Biosciences) were cloned into pLEX307 backbone (Addgene #41392) using Gateway LR II Clonase Enzyme mix (Invitrogen, 11791020) and transformed into NEB 10-beta high-efficiency competent cells

The data posited decreased caspase 3/7 activity, implying a possible defense against cytokine-induced apoptosis

Conversely, an additional experiment on illuminating the cytokine levels was carried out and the results are presented in Figure 7