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doi: 10.1038/nature23449 114 GluckSGueyBGulenMFWolterKKangTWSchmackeNAet al
Not Always Necessary For mild deficiency without absorption issues, injections may be more aggressive than required

The supernatants were then removed and the formazan dye was dissolved in dimethyl sulfoxide (DMSO)

This longer duration supports its role in long-term weight management
