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Notably, lignans exhibit anti-cancer activity through multiple regulatory pathways ( 3 Factors affecting the appropriate extraction methods for phenolic compounds Polyphenols comprise a broad array of chemical structures, resulting in varied chemical and physical properties ( Furthermore, extraction efficiency is highly sensitive to operational parameters such as pH, type of solvent employed, solvent-to-sample ratio, and duration of the extraction process ( 3.1 Extraction methods of phenolic compounds 3.1.1 Ultrasound-assisted extraction method Ultrasound-assisted extraction is a widely used and efficient technique for isolating phenolic compounds, offering high yields in a relatively short time ( The process relies on acoustic cavitation, wherein ultrasonic waves disrupt plant cell walls by inducing rapid expansion and contraction of solid surfaces ( Randia monantha , mango seed kernels, olive pomace, and pine needles ( Ultrasound-assisted extraction provides notable advantages for the extraction of polyphenols

The limit of quantification (LOQ) was as low as 10 pM, providing sufficient sensitivity for real sample analysis even after significant dilution (10-100 times)

Arch Biochem Biophys 503:24852 Zhu X, Gallogly MM, Mieyal JJ, Anderson VE, Sayre LM (2009) Covalent cross-linking of glutathione and carnosine to proteins by 4-oxo-2-nonenal

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