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Methods Enzymol 113:548555 Barhoumi R, Bailey RH, Burghardt RC (1995) Kinetic-analysis of glutathione in anchored cells with monochlorobimane

To optimize SeNPs concentration for subsequent semen cryopreservation, three semen samples were individually split into four equals and diluted to a final concentration of 6.0 10 7 spermatozoa/ml with the basic extender supplemented with 0 g/ml SeNPs, 1 g/ml SeNPs, 2 g/ml SeNPs, and 4 g/ml SeNPs

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