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Description
Lerner-Ellis, J.P., J.C
This fragment was digested with EcoRI (NEB) and ligated into pGex-6p1 (GE Healthcare) prepared by digestion with BamHI and blunting with Mung Bean Nuclease (NEB) to generate pJB-GST-HTS
A 5-unit air bubble in a 25-unit draw means you are only injecting 20 units of actual solution
If you are in pain or anxious, you may also be given medications through the IV for sedation during the procedure
