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Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline

Cleavage of the purification tag was performed using thrombin (Avantor) overnight at 4C while dialyzing into SEC buffer (50 mM NaCl, 20 mM TrisHCl 7.5, 25 mM choline chloride, 0.4 mM TCEP, 0.02% DDM, 0.002% CHS)

In addition, small sample sizes (e.g., some studies included fewer than 30 cases) may reduce statistical power and hamper the ability to effectively detect differences between patients and healthy controls

Another limitation was using a relatively moderate dose of ALC and the relatively short therapy period