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[13] Most mammalian isoenzymes have affinity for the substrate 1-chloro-2,4-dinitrobenzene, and spectrophotometric assays utilising this substrate are commonly used to report GST activity

This effect is coincident with reduced abundance of the glycolytic enzymes, aldolase A, PFK-1, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glucose-6-phosphate isomerase (GPI), lactate dehydrogenase (both LDHA and LDHB), and the serine metabolic enzyme, phosphoglycerate dehydrogenase (PHGDH)

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Because the binding affinity between (C19)x(Ub) and UT3 was determined as 9.23 M, the binding energy G is calculated as -6.87 kcal/mol ( Fig