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Following ultrasonic disruption of the cells, the cell lysates were centrifuged at 15,000 rpm for 20 min at 4 C

doi: 10.3892/or.2018.6667 216 GreenbergerLMHorakIDFilpulaDSapraPWestergaardMFrydenlundHFet al

In rats, after brain ischemia, Zn accumulated in the hippocampal hilus and CA1, as well as in the cerebral cortex, thalamus, striatum, and amygdala, while Zn chelation showed reduced ischemic neuronal degeneration (273)
