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In vitro epithelial infection model For our infections, the medium was removed and replaced with prewarmed fresh RPMI-1640 (or RPMI-1640 with L-glutamine but without glucose, catalog number 11879020, Thermo Fisher Scientific) without FBS, and supplemented with or without 10 mg/mL albumin

Another warning sign is a crinkled leaf, which develops on young leaves, stems, and petiole tissue

doi: 10.1021/jacs.5b13153 201 PetrallaS.PanayotovaM.FranchinaE.FrickerG.PurisE

Though this is a simplification with respect to the more complete Goldman-Hodgkin-Katz (GHK) flux formalism, this is sufficient for the qualitative purpose of the present investigation