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Interestingly, by similarly inhibiting specific pathway components, it was shown that this signaling was dependent on G subunits derived from D2Rs G i/o , stimulating PLC activity

However, the addition of iodomethylcholine, a gut microbial choline TMA-lyase mechanism-based inhibitor, significantly reduced the plasma level of TMAO, ameliorated renal function and inhibited renal tubulointerstitial fibrosis in isoproterenol-induced CKD rats

After sacrifice, intestine was extracted and mounted in 1% low-melting agarose for imaging