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In addition, miRNA-27a upregulation decreased the protein expression of frizzled class receptor 7 (FZD7) without changing the mRNA levels inBEL-7402/5-FU cell lines, and the use of RNA interference to decrease FZD7 protein expression was able to induce miRNA-27a-like inhibitory responses against P-gp and beta-catenin (133)

Finally, the skin used for transdermal delivery was collected and washed repeatedly with PBS

In order to verify whether ferroptosis occurred in 4T1 cells and B16 cells, we pretreated cells with ferrostatin-1 (Fer-1, 0.5 M), a ferroptosis inhibitor that prevents the formation of lipid peroxides via a reductive mechanism [51], 24 h before NSBSO treatment

doi: 10.1016/j.ophtha.2017.07.022 29