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After obtaining the Cq values, the relative expression levels of the genes are calculated using -ACTIN and GAPDH as reference genes with the 2^ Ct method

10.1007/s00380-016-0841-y Heart Vessels 40 EttingerG.MacDonaldK.ReidG.BurtonJ

acetyl-CoA is the primary substrate for the Krebs cycle, once it is de-acetylated, it must be re-charged with an acetyl-group in order for the Krebs cycle to keep working
