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Protein expression Colonies of Escherichia coli BL21 (DE3) harboring expression clones encoding recombinant GSTs M4-4, P1-1 and S1-1 were grown overnight in LB medium with 100 g ml 1 ampicillin, in a shaking incubator at 200 rpm, 37 C
While supplementing with large doses of B12 will reduce MMA levels more than will smaller doses, there are no clear benefits (more info in Appendix A
4 It remains unclear whether such extreme weight loss is mandatory to achieve an oncologic benefit, according to Ligibel, or if more moderate reductions are sufficient
