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Oxidized GSH (GSSG) is reduced back to GSH by GSR in an NADPH-dependent manner, thereby sustaining cellular antioxidant defense and linking redox buffering to central metabolic pathways that generate NADPH, such as the PPP and IDH-dependent reactions
Relative gene expression was calculated using the 2^ Ct method, where Ct = Ct of target gene Ct of reference gene, and Ct = Ct of experimental group mean Ct of control group
