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Similar to oral ethanol consumption, endogenous ethanol production has been implicated in the development of non-alcohol-associated fatty liver disease, NAFLD (now known as metabolic dysfunction-associated steatotic liver disease, MASLD), and non-alcohol-associatedsteatohepatitis NASH (now metabolic dysfunction-associated steatohepatitis, MASH) (54, 5863)

Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques

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