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Hepatocyte-like cell maturation and maintenance For small molecule HLC maturation, hepatoblasts were incubated in maturation medium containing L-15 Leibovitz medium (Thermo Fisher Scientific), 100 mM Dihexa (DC Chemicals), 3% Insulin-Transferrin-Selenium supplement (Thermo Fisher Scientific), 10% Fetal Bovine Serum (Thermo Fischer Scientific), 2 mM GlutaMAX (Thermo Fisher Scientific), 10% Tryptose Phosphate Broth (Thermo Fisher Scientific), 10 M hydrocortisone-21-hemisuccinate (Sigma), 100 nM Dexamethasone (Sigma) and 50 g/mL Sodium-L-Ascorbate (Sigma)

Gut-related applications often show improvement within 1-3 weeks

Most of the foundational studies on DSIP and Epitalon were conducted decades ago, primarily in European and Russian research institutions

Welfare of animals were considered during experiments