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Francisco V, Pino J, Gonzlez-Gay M, Lago F, Karppinen J, Tervonen O, Mobasheri A, Gualillo O
Early work in perfused rat heart preparations suggested that ergothioneine protected against ischemia-induced (oxidative) myocardial damage (Reference Arduini, Eddy and Hochstein83) , which was supported by later studies showing ergothioneine protects against reperfusion injury in rat liver (Reference Bedirli, Sakrak and Muhtaroglu84) and intestinal (Reference Sakrak, Kerem and Bedirli85) ischemic reperfusion models
After microscopic examination, oocytes at metaphase II stages (MII, with the polar body extrusion) were collected and cultured in 500 l of fertilization droplets as SOF medium supplemented with 5% fetal bovine serum, 2 mM sodium pyruvate, 10 g/ml heparin sodium and 1 mM caffeine in a CO 2 incubator (38.5C, 90%N 2 , 5%CO 2 and 5%O 2 ) (32)
