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This is due to a reduction of gastric secretions over time

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We determined cell viability and cytotoxicity by using tetrazolium reduction assay and Lactate Dehydrogenase (LDH) assay, performed real-time PCR and protein quantification to assess the expression of biomarkers after treating cells with copper peptide (GHK-Cu), copper chloride (CuCl 2 ) and copper acetate (Cu(OAc) 2 )

The first-round PCR was performed in a 40 l volume, containing 1.0 g of template DNA, 1 l each of 10 M forward primer KI#169 and 10 M reverse primer KI#289, 0.4 l of Phusion High-Fidelity DNA Polymerase (NEB, no