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The ratio of GSH (reduced) and its disulfide, GSSG (oxidized), contributes to the redox potential of the cell and thereby contributes to redox homeostasis (Fig

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While insulin resistance per se which allows glucose levels in the blood to rise to dangerous levels hasnt been found in chronic fatigue syndrome (ME/CFS), an exercise-related insulin resistance which was ameliorated by metformin did show up in cultured muscle cells from ME/CFS patients

Thirty microliters of internal standard solution were added to 30 L of each sample, vortexed for 1 min, and dried in a SpeedVac for 5 h