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While vitamin B 12 deficiency is not a primary test in newborn screening programs, a study showed that markers C3, C3/C2, and C3/C16 exhibit a negative correlation with maternal levels of vitamin B 12 in the first trimester of pregnancy, and thus may bear diagnostic value (Dayaldasani et al., 12 insufficiency or deficiency during the first trimester of pregnancy and adequate treatment

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On the other hand, the storage at room temperature (2025 C) in daylight, mimicking the procedure of commercial retailing, led to folate losses of 27% and 38% after 1 and 3 days, respectively 49