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doi: 10.1056/NEJM199704243361704
After 5 min at 95C followed by centrifugation for 10 min at 12,000 g, 20 L of the resulting supernatant was diluted with 80 L deionized water for subsequent aldehyde determination
Compared to the adult heart, oxidative damage (oxidative protein modification [49]) and calcium-mediated damage [53] are increased, indicating mitochondria-driven mechanisms of injury
Disturbance in the metabolism and homeostasis of main inhibitory and excitatory neurotransmitters, glutamate and GABA, are important pathogenic pathways
