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Metabolites and Neurotransmitters Polar hydrophilic metabolites were isolated from frontal cortex homogenates, purified by using protein and phospholipids cartridges, and finally measured by using HILIC coupled to high-resolution tandem mass spectrometry according to the nature and duration of the intervention study

For optimal study accuracy, complete your testing timeline within 21 to 30 days of mixing [verify with brand/source]
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Following a 12 h incubation period, the 143B cells were incubated with PBS, Ru(II)-OH, NP2, or NP4 (10 M Ru) for 6 h