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10.1016/j.jconrel.2023.06.005 81 SchneebergerS.KimS

Next, cDNA was synthesized by reverse transcription using the QuantiTect Reverse Transcription Kit from Qiagen according to the manufacturers instructions, and qRT-PCR was finally performed using a QuantiTect SYBR Green PCR Kit and QuantiTect Primer Assays (Qiagen) on a LightCycler 480 instrument (Roche)

Others prefer morning injection so the worst nausea hits during the day when they can actively manage it with food and movement