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For 3D cultures, Promega recommends CellTiter-Glo 3D Cell Viability Assay, which uses an enhanced lysis buffer specifically formulated to penetrate 3D tumor spheroid structures and release ATP from cells throughout the spheroid

2013 Jun;87(11):6296-305

Shamir, S

Zeng F, Nijiati S, Tang L, Ye J, Zhou Z, Chen X