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Cells were incubated with PEI-Coated-MNPs-CRISPR/Cas9 plasmid complexes (1 g DNA/well) in serum free media for 30 min followed by addition of 10% FBS via magnetic assisted transfection (magnetofection), in which a magnet was placed underneath the cells for 1 h to facilitate the localization of MNPs to the adhered cells during in vitro gene delivery

Glutathione is a redox molecule, that is, it can be in oxidized form and reduced form

With an estimated loss of roughly 90% of our global crop diversity, conserving historical crops is more important than ever - not only as a way to connect with our heritage, but to help create a more sustainable and secure food system by preserving crop diversity

These results may suggest that down-regulation of the LCY-b2 gene, resulting in inhibition of carotenoid biosynthesis, was partly responsible for the yellowing phenotype of TRV- LCYb1 + b2 tomato plants