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Frataxin deficiency impairs mitochondrial biogenesis in cells, mice and humans

Hormone Replacement Therapy (HRT) A growing number of studies indicate that estrogen therapy has a broad spectrum of beneficial effects in post-menopausal women (Behl and Lezoualch, 1998

Key benefits of this recovery protocol include: Accelerated Soft Tissue Repair : Speeds up the healing of muscle tears, ligament sprains, and tendon injuries

Protein preparation and Western blot analysis Cultured cells or flashfrozen tissues were homogenized in icecold radioimmunoprecipitation assay (RIPA) buffer (50 mM TrisHCl pH 7.6, 150 mM NaCl, 0.5% sodium deoxycholate, 1% NP40, 0.1% SDS, 2 mM EDTA) containing COMPLETE protease inhibitor cocktail (Roche) and phosphatase inhibitors (Santa Cruz Biotechnology)