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The lower chambers were filled with 600 l DMEM containing 10% fetal bovine serum

The proton NMR spectra of the complexes in DMSO- d 6 show the typical signal of the NH 3 protons which is a multiplet at around 6 ppm for the monocarboxylato complexes 1P and 1A (typical 1 J NH and 2 J PtH values around 50 Hz) while it is a broad signal around 6.5 ppm for the bis-substituted complexes ( 2 5A and 2 5P , Supplementary Information) 44

Then, it critically appraises the published evidence, focusing on studies conducted over the last 5 years, regarding the potential atheroprotective effects of methotrexate in experimental and clinical studies in patients with and without autoimmune and inflammatory conditions

Total prevention of the development of in vitro tolerance to organic nitrates