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Description
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A filtered gas line connected to a gas tank containing 1% O 2 , 5% CO 2 , and 94% N 2 gas mixture was placed inside the flask for about 45 seconds to induce the deoxygenated environment needed for parasite growth

Wuillemin et al., 1996) and prevented the deposition of C3 and C4 (Wuillemin et al., 1997)
