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Subsequently, sdAb-GEM-IRDye800CW (2 nmol or 12 nmol of protein for in vitro and in vivo experiments, respectively) were incubated with [[ 99m Tc]Tc(H 2 O) 3 (CO) 3 ] + (activities ranging between 0 and 185 MBq/nmol) in PBS (250-500 l, pH 7.4) at 50C for 90 min

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