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This reagent reacts with the thiol groups of GSH which convert 2-nitro-5-thiobenzoate (NTB ) to NTB 2 , thereby producing a yellow color, which can subsequently be quantified ( Glutathione Peroxidase GPx is measured by adding glutathione reductase (GR) to the sample after which nicotinamide adenine dinucleotide phosphate (NADPH) is transformed to a reduced state, allowing a decrease in absorbance to be subsequently measured (32)

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Possible reasons could be that (1) DAB is preferred by LaccID over other aromatic substrates, and (2) DAB labeling was performed in sodium cacodylate buffer, which does not contain laccase inhibitors such as halides and thiols