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Step 1: Hydration is Key The first of the four steps starts with general supplements and hydration : thiamine 600 1800 mg oral rehydration salts 20 mg in 1 L of water twice per week N-acetyl cysteine, multi-vitamin, and multi-mineral including potassium and magnesium The idea for thiamine (vitamin B1) came from a Danish paper by Bager and colleagues showing that people in remission from inflammatory bowel disease, yet still experiencing significant fatigue, improved when given high dose thiamine for 4 weeks

The normalized protein intensity of each identified peptide was used for label-free quantitation using the LFQ-MBR workflow