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Description
The eluted fractions were pooled, their protein concentration determined by using bicinchoninic acid (BCA) protein assay (Pierce) and the samples were digested using the filter-aided sample preparation (FASP) method (Wisniewski et al, 2009)

In our constantly evolving world, these neurons need constant renewal in order to keep up with developments

Each vial yields 1819 practical doses at 500mcg per administration, meaning you need 5 vials minimum (5 18 = 90 doses, covering 84 days with 6-dose buffer)

Lagergren, J., Bergstrom, R., Lindgren, A
