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Thus, passive diffusion of peptides is very limited unless the peptides possess an amphipathic structure or use synthetic means to modify the lipophilicity [2,6], such as the glycosylation strategy of peptides [12]

The inhibition of XO in the presence of bovine serum albumin was also evaluated, where the IC 50 values of 30 and 31 showed a slight worsening in activity, while 32 showed a slight improvement in activity

General Chemicals Used for This Study Glutathione, Glutathione disulfide (GSSG), antibiotics (ciprofloxacin, amikacin, Augmentin, gentamicin and tobramycin), resazurin dye, crystal violet solution, Phosphate buffered saline (1 PBS) and Hydrogen peroxide (H 2 O 2 ) were all obtained from Sigma-Aldrich (Sydney, Australia)

pH-Dependent hydrolase, glutaminase, transpeptidase and autotranspeptidase activities of Bacillus subtilis -glutamyltransferase