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The volume math assumes the bac water is on-spec 0.9% benzyl alcohol, sterile, non-pyrogenic

Mixed Mode Tests (HAT/SET) These mixed mode tests are generally based on the elimination of a stable chromophore (like 2,2-azinobis-3-ethylbenzthyazolin-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazil (DPPH)), where HAT, ET, and proton-coupled electron transfer (PCET) mechanisms may play different roles in varied proportions, depending on the corresponding reaction conditions (such as pH and solvent) [66]

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One of the earliest papers describing a reproducible method for developing a BBB co-culture model with brain endothelial and glial cells that could be used in the pharmaceutical industry