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Peptide identification Raw data files were analyzed using MaxQuant (development version 1.5.2.8) 39

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This suggests that GluK3 possesses at least two functionally distinct zinc-binding sites: (1) the high-affinity potentiating site at the LBD dimer interface involving D759, and (2) a lower-affinity, inhibitory site whose action is normally overshadowed by the dominant potentiation