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Front Physiol 2019;10:70

When skin skews towards an alkaline pH, it can become dry and show signs of premature ageing such as fine lines, wrinkles, and hyperpigmentation

Reagents The following antibodies and their corresponding Research Resource Identifiers (RRIDs) were used in this study: SLC25A39 (RRID: AB_2878095, AB_2605624), SLC25A40 (RRID: AB_1310678), AFG3L2 (RRID: AB_2637746, AB_1209606), HSP60 (RRID: AB_881444), -actin (RRID: AB_10950489), GAPDH (RRID: AB_307272), cleaved caspase-3 (RRID: AB_302962), cleaved caspase-9 (RRID: AB_2889070), cleaved PARP (RRID: AB_777102), HEY1 (RRID: AB_2576326), pro-caspase-3 (RRID: AB_2069711), pro-caspase-9 (RRID: AB_2809709), HES1 (RRID: AB_2546278), and NOTCH1 (RRID: AB_467579)

Overall, whilst these findings imply that increased production of ROS is associated with C9orf72-ALS, it is unclear whether loss of C9orf72 or expression of hexanucleotide repeat expansions disturbs redox homeostasis