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Lipotropic injections include B12 plus additional fat-metabolizing compounds like methionine, inositol, choline, and other B vitamins

PLoS ONE 7 , e31882 (2012)

Confocal microscopy For confocal microscopy assay, cells were washed twice with a washing buffer (D-PBS supplemented with 4.5 g/L of glucose and 5 mM of MgCl 2 ), followed by treatment with Cy5-labeled AsiG (250 nM) either free or loaded by liposome with or without anti-CD71 antibody in a binding buffer (D-PBS supplemented with 4.5 g/L of glucose, 5 mM of MgCl 2 , 1 mg/mL BSA, and 0.1 mg/mL of yeast tRNA) for 1 h at pH 7.4 or 6.5 on ice

This mechanism also produces 5-S-cysteinyl dopamine in the brain, where it exerts neurotoxic activity [20]