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Harvested cells were sonicated in TBS buffer (20 mM Tris pH 8.0, 150 mM NaCl) and solubilized in 200 uL of lysis buffer (20 mM Tris pH 8.0, 150 mM NaCl, 1% DDM, 1 mM PMSF) for 2 hrs

International regulations vary in some countries, it may be classified as an unlicensed medicine or research chemical

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